Job Title: UAF in Correlative Light and Electron Microscopy
Juan Fontana obtained his Ph.D. at the Cell Structure Laboratory (National Center for Biotechnology, Spanish Council for Scientific Research, Madrid, Spain). During this period, he characterized the replication complexes formed by Bunyamwera and Rubella viruses, both of which replicate their genome in cytoplasmic viral factories. As a post-doctoral researcher at the Laboratory of Structural Biology Research (National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA), his research was focused in enveloped virus entry and morphogenesis. Specifically, he analyzed the structural intermediates of Influenza Virus hemagglutinin and M1 matrix proteins prior to viral fusion; he studied the two-step fusion process of the retrovirus Avian Sarcoma/Leukosis, in which receptor binding necessarily precedes low-pH activation of the fusion protein; and he analyzed the effect of several drugs and mutations on HIV core maturation. Additionally, he obtained a 4.6 Å resolution cryo-electron microscopy reconstruction of the shell protein of a bacterial protein-bound nanocompartment, which led to an atomic model in which the HK97 fold (from the HK97 bacteriophage capsid protein) is evident. Dr. Fontana will be joining the School of Molecular and Cellular Biology in early 2016, and his research will be focused on understanding virus entry and morphogenesis. To this end he will take advantage of his expert knowledge of cellular and cryo-electron microscopy techniques.
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Research Gate: https://www.researchgate.net/profile/Juan_Fontana3